Dear customer, please register and verify your account so you can see product price, discounts and make inquiries. Click here to register.
Euro ADA Adenosine Deaminase Diagnostic Reagent
Euro
Dear customer, please register your account with us to know more about price and discount offers on product. Click here to register.
| Brand | Euro |
| Country of Origin | Made in India |
R1 R2 Cal Control | 1 x 20 ml 1 x 10 ml 1 x 1 ml 1 x 2 ml |
Product Description :
Clinical Significance ADA is an enzyme catalyzing the deamination reaction from adenosine to inosine. The enzyme is widely distributed in human tissues, especially high in T lymphocytes. Elevated serum ADA activity has been observed in patients with acute hepatitis, alcoholic hepatic fibrosis, chronic active hepatitis, liver cirrhosis, viral hepatitis and hepatoma 1,2. Increased ADA activity was also observed in patients with tuberculous effusions 3 . Determination of ADA activity in patient serum may add unique values to the diagnosis of liver diseases in combination with ALT or γ-GT (GGT) tests. ADA assay may also be useful in the diagnostics of tuberculous pleuritis 3 .
Principle: The ADA assay is based on the enzymatic deamination of adenosine to inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with TOOS and 4-aminoantipyrine (4-AA) in the presence of peroxidase (POD) to generate quinone dye which is monitored in a kinetic manner.
Precautions: Solution R1 and CAL contain Sodium Azide. Avoid ingestion or contact with skin or mucous membranes. In case of skin contact, flush affected area with copious amounts of water. In case of contact with eyes or if ingested, seek immediate medical attention. All specimens used in this test should be considered potentially infectious.
Calibration: Recommend that this assay should be calibrated using the ADA CAL (ADA Calibrator) included in the kit. Reagent deterioration: Presence of particles and turbidity.
ada euro peerless adenosine deaminase ada 30 ml euro
